Summary
Type: SPE
Application #: 300370
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Conditioning of the 1st column:
1 column volume methanol, then 1 column volume methanol / water (8 : 2, v/v)
Sample application:
Slowly force or aspirate 3 ml homogenised unfiltered flour through the 1st
column. Dilute 2 ml of the eluate to 5 ml with dist. water
Conditioning of the 2nd column:
1 column volume methanol, then 1 column volume dist. water
Sample application:
Slowly force or aspirate 5 ml of the diluted eluate from the 1st column through
the 2nd column
Column washing:
1 column volume dist. water, then 1 ml n-hexane; dry column under vacuum for 5
min
Elution:
3 x 500 μl methylene chloride
Authors: LC department
Source: Macherey-Nagel